James Timberlake, American lieutenant, police officer, and farmer (d. 1891)

James H. Timberlake (March 22, 1846 – February 21, 1891) was an American law enforcement officer, Civil War soldier, farmer and rancher who served as a deputy U.S. marshal for the Western District of Missouri. Timberlake is best known for being the chief enforcer and investigator against the James-Younger Gang, beginning in the 1870s, which culminated in the death of the outlaw Jesse James on April 3, 1882, at the hands of Robert Ford.