Space Shuttle Columbia (OV-102) was a Space Shuttle orbiter manufactured by Rockwell International and operated by NASA. Named after the first American ship to circumnavigate the upper North American Pacific coast and the female personification of the United States, Columbia was the first of five Space Shuttle orbiters to fly in space, debuting the Space Shuttle launch vehicle on its maiden flight in April 1981. As only the second full-scale orbiter to be manufactured after the Approach and Landing Test vehicle Enterprise, Columbia retained unique features indicative of its experimental design compared to later orbiters, such as test instrumentation and distinctive black chines. In addition to a heavier fuselage and the retention of an internal airlock throughout its lifetime, these made Columbia the heaviest of the five spacefaring orbiters; around 1,000 kilograms (2,200 pounds) heavier than Challenger and 3,600 kilograms (7,900 pounds) heavier than Endeavour. Columbia also carried ejection seats based on those from the SR-71 during its first six flights until 1983, and from 1986 onwards carried an external scientific instrument bay on its vertical stabilizer.

During its 22 years of operation, Columbia was flown on 28 missions in the Space Shuttle program, spending over 300 days in space and completing over 4,000 orbits around Earth. While it was seldom used after completing its objective of testing the Space Shuttle system, and its heavier mass and internal airlock made it unideal for planned Shuttle-Centaur launches and dockings with space stations, it nonetheless proved useful as a workhorse for scientific research in orbit following the loss of Challenger in 1986. Columbia was used for eleven of the fifteen flights of Spacelab laboratories, all four United States Microgravity Payload missions, and the only flight of Spacehab's Research Double Module. The Extended Duration Orbiter pallet was used by the orbiter in thirteen of the pallet's fourteen flights, which aided lengthy stays in orbit for scientific and technological research missions. Columbia was also used to retrieve the Long Duration Exposure Facility and deploy the Chandra observatory, and also carried into space the first female commander of an American spaceflight mission, the first ESA astronaut, the first female astronaut of Indian origin, and the first Israeli astronaut.

At the end of its final flight in February 2003, Columbia disintegrated upon reentry, killing the seven-member crew of STS-107 and destroying most of the scientific payloads aboard. The Columbia Accident Investigation Board convened shortly afterwards concluded that damage sustained to the orbiter's left wing during the launch of STS-107 fatally compromised the vehicle's thermal protection system. The loss of Columbia and its crew led to a refocusing of NASA's human exploration programs and led to the establishment of the Constellation program in 2005 and the eventual retirement of the Space Shuttle program in 2011. Numerous memorials and dedications were made to honor the crew following the disaster; the Columbia Memorial Space Center was opened as a national memorial for the accident, and the Columbia Hills in Mars' Gusev crater, which the Spirit rover explored, were named after the crew. The majority of Columbia's recovered remains are stored at the Kennedy Space Center's Vehicle Assembly Building, though some pieces are on public display at the nearby Visitor Complex.

The Space Shuttle is a retired, partially reusable low Earth orbital spacecraft system operated from 1981 to 2011 by the U.S. National Aeronautics and Space Administration (NASA) as part of the Space Shuttle program. Its official program name was Space Transportation System (STS), taken from a 1969 plan for a system of reusable spacecraft where it was the only item funded for development. The first (STS-1) of four orbital test flights occurred in 1981, leading to operational flights (STS-5) beginning in 1982. Five complete Space Shuttle orbiter vehicles were built and flown on a total of 135 missions from 1981 to 2011, launched from the Kennedy Space Center (KSC) in Florida. Operational missions launched numerous satellites, interplanetary probes, and the Hubble Space Telescope (HST), conducted science experiments in orbit, participated in the Shuttle-Mir program with Russia, and participated in construction and servicing of the International Space Station (ISS). The Space Shuttle fleet's total mission time was 1,323 days.Space Shuttle components include the Orbiter Vehicle (OV) with three clustered Rocketdyne RS-25 main engines, a pair of recoverable solid rocket boosters (SRBs), and the expendable external tank (ET) containing liquid hydrogen and liquid oxygen. The Space Shuttle was launched vertically, like a conventional rocket, with the two SRBs operating in parallel with the orbiter's three main engines, which were fueled from the ET. The SRBs were jettisoned before the vehicle reached orbit, while the main engines continued to operate, and the ET was jettisoned after main engine cutoff and just before orbit insertion, which used the orbiter's two Orbital Maneuvering System (OMS) engines. At the conclusion of the mission, the orbiter fired its OMS to deorbit and reenter the atmosphere. The orbiter was protected during reentry by its thermal protection system tiles, and it glided as a spaceplane to a runway landing, usually to the Shuttle Landing Facility at KSC, Florida, or to Rogers Dry Lake in Edwards Air Force Base, California. If the landing occurred at Edwards, the orbiter was flown back to the KSC atop the Shuttle Carrier Aircraft (SCA), a specially modified Boeing 747.

The first orbiter, Enterprise, was built in 1976 and used in Approach and Landing Tests (ALT), but had no orbital capability. Four fully operational orbiters were initially built: Columbia, Challenger, Discovery, and Atlantis. Of these, two were lost in mission accidents: Challenger in 1986 and Columbia in 2003, with a total of 14 astronauts killed. A fifth operational (and sixth in total) orbiter, Endeavour, was built in 1991 to replace Challenger. The three surviving operational vehicles were retired from service following Atlantis's final flight on July 21, 2011. The U.S. relied on the Russian Soyuz spacecraft to transport astronauts to the ISS from the last Shuttle flight until the launch of the Crew Dragon Demo-2 mission in May 2020.