Apollo 16 (April 1627, 1972) was the tenth crewed mission in the United States Apollo space program, administered by NASA, and the fifth and penultimate to land on the Moon. It was the second of Apollo's "J missions", with an extended stay on the lunar surface, a focus on science, and the use of the Lunar Roving Vehicle (LRV). The landing and exploration were in the Descartes Highlands, a site chosen because some scientists expected it to be an area formed by volcanic action, though this proved to not be the case.

The mission was crewed by Commander John Young, Lunar Module Pilot Charles Duke and Command Module Pilot Ken Mattingly. Launched from the Kennedy Space Center in Florida on April 16, 1972, Apollo 16 experienced a number of minor glitches en route to the Moon. These culminated with a problem with the spaceship's main engine that resulted in a six-hour delay in the Moon landing as NASA managers contemplated having the astronauts abort the mission and return to Earth, before deciding the problem could be overcome. Although they permitted the lunar landing, NASA had the astronauts return from the mission one day earlier than planned.

After flying the lunar module to the Moon's surface on April 21, Young and Duke spent 71 hoursjust under three dayson the lunar surface, during which they conducted three extravehicular activities or moonwalks, totaling 20 hours and 14 minutes. The pair drove the lunar rover, the second used on the Moon, for 26.7 kilometers (16.6 mi). On the surface, Young and Duke collected 95.8 kilograms (211 lb) of lunar samples for return to Earth, including Big Muley, the largest Moon rock collected during the Apollo missions. During this time Mattingly orbited the Moon in the command and service module (CSM), taking photos and operating scientific instruments. Mattingly, in the command module, spent 126 hours and 64 revolutions in lunar orbit. After Young and Duke rejoined Mattingly in lunar orbit, the crew released a subsatellite from the service module (SM). During the return trip to Earth, Mattingly performed a one-hour spacewalk to retrieve several film cassettes from the exterior of the service module. Apollo 16 returned safely to Earth on April 27, 1972.

The Apollo program, also known as Project Apollo, was the third United States human spaceflight program carried out by the National Aeronautics and Space Administration (NASA), which succeeded in preparing and landing the first humans on the Moon from 1968 to 1972. It was first conceived during Dwight D. Eisenhower's administration as a three-person spacecraft to follow the one-person Project Mercury, which put the first Americans in space. Apollo was later dedicated to President John F. Kennedy's national goal for the 1960s of "landing a man on the Moon and returning him safely to the Earth" in an address to Congress on May 25, 1961. It was the third US human spaceflight program to fly, preceded by the two-person Project Gemini conceived in 1961 to extend spaceflight capability in support of Apollo.

Kennedy's goal was accomplished on the Apollo 11 mission when astronauts Neil Armstrong and Buzz Aldrin landed their Apollo Lunar Module (LM) on July 20, 1969, and walked on the lunar surface, while Michael Collins remained in lunar orbit in the command and service module (CSM), and all three landed safely on Earth on July 24. Five subsequent Apollo missions also landed astronauts on the Moon, the last, Apollo 17, in December 1972. In these six spaceflights, twelve people walked on the Moon.

Apollo ran from 1961 to 1972, with the first crewed flight in 1968. It encountered a major setback in 1967 when an Apollo 1 cabin fire killed the entire crew during a prelaunch test. After the first successful landing, sufficient flight hardware remained for nine follow-on landings with a plan for extended lunar geological and astrophysical exploration. Budget cuts forced the cancellation of three of these. Five of the remaining six missions achieved successful landings, but the Apollo 13 landing was prevented by an oxygen tank explosion in transit to the Moon, which destroyed the service module's capability to provide electrical power, crippling the CSM's propulsion and life support systems. The crew returned to Earth safely by using the lunar module as a "lifeboat" for these functions. Apollo used the Saturn family of rockets as launch vehicles, which were also used for an Apollo Applications Program, which consisted of Skylab, a space station that supported three crewed missions in 1973–1974, and the Apollo–Soyuz Test Project, a joint United States-Soviet Union low Earth orbit mission in 1975.

Apollo set several major human spaceflight milestones. It stands alone in sending crewed missions beyond low Earth orbit. Apollo 8 was the first crewed spacecraft to orbit another celestial body, and Apollo 11 was the first crewed spacecraft to land humans on one.

Overall the Apollo program returned 842 pounds (382 kg) of lunar rocks and soil to Earth, greatly contributing to the understanding of the Moon's composition and geological history. The program laid the foundation for NASA's subsequent human spaceflight capability, and funded construction of its Johnson Space Center and Kennedy Space Center. Apollo also spurred advances in many areas of technology incidental to rocketry and human spaceflight, including avionics, telecommunications, and computers.