The Treaty of Greenville, formally titled Treaty with the Wyandots, etc., was a 1795 treaty between the United States and indigenous nations of the Northwest Territory (now Midwestern United States), including the Wyandot and Delaware peoples, that redefined the boundary between indigenous peoples' lands and territory for European American community settlement.

It was signed at Fort Greenville, now Greenville, Ohio, on August 3, 1795, following the Native American loss at the Battle of Fallen Timbers a year earlier. It ended the Northwest Indian War in the Ohio Country, limited Indian Country to northwestern Ohio, and began the practice of annual payments following the land concessions. The parties to the treaty were a coalition of Native American tribes known as the Western Confederacy, and the United States government represented by General Anthony Wayne and local frontiersmen.

The treaty became synonymous with the end of the frontier in that part of the Northwest Territory that would become the new state of Ohio.