The Daytona 500 is a 500-mile-long (805 km) NASCAR Cup Series motor race held annually at Daytona International Speedway in Daytona Beach, Florida. It is the first of two Cup races held every year at Daytona, the second being the Coke Zero Sugar 400, and one of three held in Florida, with the annual fall showdown Dixie Vodka 400 being held at Homestead south of Miami. From 1988 to 2019, it was one of the four restrictor plate races on the Cup schedule. The inaugural Daytona 500 was held in 1959 coinciding with the opening of the speedway and since 1982, it has been the season-opening race of the Cup series.The Daytona 500 is regarded as the most important and prestigious race on the NASCAR calendar, carrying by far the largest purse. Championship points awarded are equal to that of any other NASCAR Cup Series race. It is also the series' first race of the year; this phenomenon is unique in sports, which tend to have championships or other major events at the end of the season rather than the start. From 1995-2020, U.S. television ratings for the Daytona 500 have been the highest for any auto race of the year, surpassing the traditional leader, the Indianapolis 500 which in turn greatly surpasses the Daytona 500 in in-track attendance and international viewing; however, in 2021 the Indianapolis 500 surpassed the Daytona 500 in TV ratings and viewership. The 2006 Daytona 500 attracted the sixth largest average live global TV audience of any sporting event that year with 20 million viewers.The race serves as the final event of Speedweeks and is also known as "The Great American Race" or the "Super Bowl of Stock Car Racing". Since its inception, the race has been held in mid-to-late February. From 1971 to 2011, and again since 2018, the event has been as associated with Presidents Day weekend, taking place on the Sunday before the third Monday in February. On eight occasions, the race has been run on Valentine's Day.

Since 1997, the winner of the Daytona 500 has been presented with the Harley J. Earl Trophy in Victory Lane, and the winning car is displayed in race-winning condition for one year at Daytona 500 Experience, a museum and gallery adjacent to Daytona International Speedway.

Austin Cindric is the most recent winner of the Daytona 500, having won it in 2022.

Lee Arnold Petty (March 14, 1914 – April 5, 2000) was an American stock car racing driver who competed during the 1950s and 1960s. He was one of the pioneers of NASCAR and one of its first superstars. He was NASCAR's first three-time Cup champion. He is also the father of Richard Petty, who went on to become one of the most successful stock car racing drivers of all time.