STS-107 was the 113th flight of the Space Shuttle program, and the 28th and final flight of Space Shuttle Columbia. The mission launched from Kennedy Space Center in Florida on January 16, 2003 and during its 15 days, 22 hours, 20 minutes, 32 seconds in orbit conducted a multitude of international scientific experiments.An in-flight break up during reentry into the atmosphere on February 1 killed all seven crew members and disintegrated Columbia. Immediately after the disaster, NASA convened the Columbia accident Investigation Board to determine the cause of the disintegration. The source of the failure was determined to have been caused by a piece of foam that broke off during launch and damaged the thermal protection system (reinforced carbon-carbon panels and thermal protection tiles) on the leading edge of the orbiter's left wing. During re-entry the damaged wing slowly overheated and came apart, eventually leading to loss of control and disintegration of the vehicle. The cockpit window frame is now exhibited in a memorial inside the Space Shuttle Atlantis Pavilion at the Kennedy Space Center.

The damage to the thermal protection system on the wing was similar to that Atlantis had sustained in 1988 during STS-27, the second mission after the Space Shuttle Challenger disaster. However, the damage on STS-27 occurred at a spot that had more robust metal (a thin steel plate near the landing gear), and that mission survived the re-entry.

Space Shuttle Columbia (OV-102) was a Space Shuttle orbiter manufactured by Rockwell International and operated by NASA. Named after the first American ship to circumnavigate the upper North American Pacific coast and the female personification of the United States, Columbia was the first of five Space Shuttle orbiters to fly in space, debuting the Space Shuttle launch vehicle on its maiden flight in April 1981. As only the second full-scale orbiter to be manufactured after the Approach and Landing Test vehicle Enterprise, Columbia retained unique features indicative of its experimental design compared to later orbiters, such as test instrumentation and distinctive black chines. In addition to a heavier fuselage and the retention of an internal airlock throughout its lifetime, these made Columbia the heaviest of the five spacefaring orbiters; around 1,000 kilograms (2,200 pounds) heavier than Challenger and 3,600 kilograms (7,900 pounds) heavier than Endeavour. Columbia also carried ejection seats based on those from the SR-71 during its first six flights until 1983, and from 1986 onwards carried an external scientific instrument bay on its vertical stabilizer.

During its 22 years of operation, Columbia was flown on 28 missions in the Space Shuttle program, spending over 300 days in space and completing over 4,000 orbits around Earth. While it was seldom used after completing its objective of testing the Space Shuttle system, and its heavier mass and internal airlock made it unideal for planned Shuttle-Centaur launches and dockings with space stations, it nonetheless proved useful as a workhorse for scientific research in orbit following the loss of Challenger in 1986. Columbia was used for eleven of the fifteen flights of Spacelab laboratories, all four United States Microgravity Payload missions, and the only flight of Spacehab's Research Double Module. The Extended Duration Orbiter pallet was used by the orbiter in thirteen of the pallet's fourteen flights, which aided lengthy stays in orbit for scientific and technological research missions. Columbia was also used to retrieve the Long Duration Exposure Facility and deploy the Chandra observatory, and also carried into space the first female commander of an American spaceflight mission, the first ESA astronaut, the first female astronaut of Indian origin, and the first Israeli astronaut.

At the end of its final flight in February 2003, Columbia disintegrated upon reentry, killing the seven-member crew of STS-107 and destroying most of the scientific payloads aboard. The Columbia Accident Investigation Board convened shortly afterwards concluded that damage sustained to the orbiter's left wing during the launch of STS-107 fatally compromised the vehicle's thermal protection system. The loss of Columbia and its crew led to a refocusing of NASA's human exploration programs and led to the establishment of the Constellation program in 2005 and the eventual retirement of the Space Shuttle program in 2011. Numerous memorials and dedications were made to honor the crew following the disaster; the Columbia Memorial Space Center was opened as a national memorial for the accident, and the Columbia Hills in Mars' Gusev crater, which the Spirit rover explored, were named after the crew. The majority of Columbia's recovered remains are stored at the Kennedy Space Center's Vehicle Assembly Building, though some pieces are on public display at the nearby Visitor Complex.