The House of Burgesses was the elected representative element of the Virginia General Assembly, the legislative body of the Colony of Virginia. With the creation of the House of Burgesses in 1642, the General Assembly, which had been established in 1619, became a bicameral institution.

From 1642 to 1776, the House of Burgesses was an instrument of government alongside the royally-appointed colonial governor and the upper-house Council of State in the General House.When the Virginia colony declared its independence from the Kingdom of Great Britain at the Fifth Virginia Convention in 1776 and became the independent Commonwealth of Virginia, the House of Burgesses became the House of Delegates, which continues to serve as the lower house of the General Assembly.

The Jamestown settlement in the Colony of Virginia was the first permanent English settlement in the Americas. It was located on the northeast bank of the James (Powhatan) River about 2.5 mi (4 km) southwest of the center of modern Williamsburg. It was established by the Virginia Company of London as "James Fort" on May 4, 1607 O.S. (May 14, 1607 N.S.), and was considered permanent after a brief abandonment in 1610. It followed several failed attempts, including the Lost Colony of Roanoke, established in 1585 on Roanoke Island, later part of North Carolina. Jamestown served as the colonial capital from 1616 until 1699.

Despite the dispatch of more settlers and supplies, including the 1608 arrival of eight Polish and German colonists and the first two European women, more than 80 percent of the colonists died in 1609–10, mostly from starvation and disease. In mid-1610, the survivors abandoned Jamestown, though they returned after meeting a resupply convoy in the James River.

In August 1619, the first recorded slaves from Africa to British North America arrived in what is now Old Point Comfort near the Jamestown colony, on a British privateer ship flying a Dutch flag. The approximately 20 Africans from the present-day Angola had been removed by the British crew from the Portuguese slave ship São João Bautista. They most likely worked in the tobacco fields as slaves under a system of race-based indentured servitude. One of their number included Angela, who was purchased by William Peirce. The modern conception of slavery in the colonial United States was formalized in 1640 (the John Punch hearing) and was fully entrenched in Virginia by 1660.The London Company's second settlement in Bermuda claims to be the site of the oldest town in the English New World, as St. George's, Bermuda, was officially established in 1612 as New London, whereas James Fort in Virginia was not converted into James Towne until 1619, and further did not survive to the present day.In 1676, Jamestown was deliberately burned during Bacon's Rebellion, though it was quickly rebuilt. In 1699, the colonial capital was moved to what is today Williamsburg, Virginia; Jamestown ceased to exist as a settlement, and remains today only as an archaeological site, Jamestown Rediscovery.

Today, Jamestown is one of three locations composing the Historic Triangle of Colonial Virginia, along with Williamsburg and Yorktown, with two primary heritage sites. Historic Jamestowne is the archaeological site on Jamestown Island and is a cooperative effort by Jamestown National Historic Site (part of Colonial National Historical Park) and Preservation Virginia. Jamestown Settlement, a living history interpretive site, is operated by the Jamestown Yorktown Foundation, a state agency of the Commonwealth of Virginia.