Henry VIII (28 June 1491 28 January 1547) was King of England from 22 April 1509 until his death in 1547. Henry is best known for his six marriages, and for his efforts to have his first marriage (to Catherine of Aragon) annulled. His disagreement with Pope Clement VII about such an annulment led Henry to initiate the English Reformation, separating the Church of England from papal authority. He appointed himself Supreme Head of the Church of England and dissolved convents and monasteries, for which he was excommunicated. Henry is also known as "the father of the Royal Navy", as he invested heavily in the navy, increasing its size from a few to more than 50 ships, and established the Navy Board.Domestically, Henry is known for his radical changes to the English Constitution, ushering in the theory of the divine right of kings in opposition to Papal supremacy. He also greatly expanded royal power during his reign. He frequently used charges of treason and heresy to quell dissent, and those accused were often executed without a formal trial by means of bills of attainder. He achieved many of his political aims through the work of his chief ministers, some of whom were banished or executed when they fell out of his favour. Thomas Wolsey, Thomas More, Thomas Cromwell, Richard Rich, and Thomas Cranmer all figured prominently in his administration.

Henry was an extravagant spender, using the proceeds from the dissolution of the monasteries and acts of the Reformation Parliament. He also converted the money that was formerly paid to Rome into royal revenue. Despite the money from these sources, he was continually on the verge of financial ruin due to his personal extravagance, as well as his numerous costly and largely unsuccessful wars, particularly with King Francis I of France, Holy Roman Emperor Charles V, King James V of Scotland and the Scottish regency under the Earl of Arran and Mary of Guise. At home, he oversaw the legal union of England and Wales with the Laws in Wales Acts 1535 and 1542, and he was the first English monarch to rule as King of Ireland following the Crown of Ireland Act 1542.

Henry's contemporaries considered him to be an attractive, educated, and accomplished king. He has been described as "one of the most charismatic rulers to sit on the English throne" and his reign has been described as the "most important" in English history. He was an author and composer. As he aged, he became severely overweight and his health suffered. He is frequently characterised in his later life as a lustful, egotistical, paranoid and tyrannical monarch. He was succeeded by his son Edward VI.

Anne Boleyn (; c. 1501 – 19 May 1536) was Queen of England from 1533 to 1536, as the second wife of King Henry VIII. The circumstances of her marriage and of her execution by beheading for treason and other charges made her a key figure in the political and religious upheaval that marked the start of the English Reformation. Anne was the daughter of Thomas Boleyn, 1st Earl of Wiltshire, and his wife, Lady Elizabeth Howard, and was educated in the Netherlands and France, largely as a maid of honour to Queen Claude of France. Anne returned to England in early 1522, to marry her Irish cousin James Butler, 9th Earl of Ormond; the marriage plans were broken off, and instead she secured a post at court as maid of honour to Henry VIII's wife, Catherine of Aragon.

Early in 1523, Anne was secretly betrothed to Henry Percy, son of Henry Percy, 5th Earl of Northumberland, but the betrothal was broken off when the Earl refused to support their engagement. Cardinal Thomas Wolsey refused the match in January 1524 and Anne was sent home to Hever Castle. In February or March 1526, Henry VIII began his pursuit of Anne. She resisted his attempts to seduce her, refusing to become his mistress, as her sister Mary had previously been. Henry soon focused his desires on annulling his marriage to Catherine so he would be free to marry Anne. Wolsey failed to obtain an annulment of Henry's marriage from Pope Clement VII and, in 1529–30, Anne helped bring about his downfall and his death. When it became clear that Clement would not annul the marriage, Henry and his advisers, such as Thomas Cromwell, began the breaking of the Catholic Church's power in England and closing the monasteries and the nunneries. In 1532, Henry made Anne the Marquess of Pembroke.

Henry and Anne formally married on 25 January 1533, after a secret wedding on 14 November 1532. On 23 May 1533, the newly appointed Archbishop of Canterbury Thomas Cranmer declared Henry and Catherine's marriage null and void; five days later, he declared Henry and Anne's marriage valid. Shortly afterwards, Clement excommunicated Henry and Cranmer. As a result of this marriage and these excommunications, the first break between the Church of England and the Catholic Church took place, and the king took control of the Church of England. Anne was crowned Queen of England on 1 June 1533. On 7 September, she gave birth to the future Queen Elizabeth I. Henry was disappointed to have a daughter rather than a son but hoped a son would follow and professed to love Elizabeth. Anne subsequently had three miscarriages and by March 1536, Henry was courting Jane Seymour. In order to marry Seymour, Henry had to find reasons to end the marriage to Anne.

Henry VIII had Anne investigated for high treason in April 1536. On 2 May, she was arrested and sent to the Tower of London, where she was tried before a jury of peers, including Henry Percy, her former betrothed, and her uncle Thomas Howard, 3rd Duke of Norfolk; she was convicted on 15 May and beheaded four days later. Modern historians view the charges against her, which included adultery, incest and plotting to kill the king, as unconvincing.After her daughter, Elizabeth, was crowned as Queen in 1558, Anne became venerated as a martyr and heroine of the English Reformation, particularly through the written works of John Foxe. She has inspired, or been mentioned in, many artistic and cultural works and retained her hold on the popular imagination. She has been called "the most influential and important queen consort England has ever had", as she provided the occasion for Henry VIII to annul his marriage to Catherine of Aragon and declare the English church's independence from the Vatican.