The 1995 Fox River Grove bus–train collision was a grade crossing collision that killed seven students riding aboard a school bus in Fox River Grove, Illinois, on the morning of October 25, 1995. The school bus, driven by a substitute driver, was stopped at a traffic light with the rearmost portion extending onto a portion of the railroad tracks when it was struck by a Metra Union Pacific / Northwest Line train en route to Chicago.The crash involved a signalled rail crossing located very near a highway intersection which was regulated by traffic signals. The devices were connected and operations were supposed to be carefully timed and coordinated. Such locations are known as "interconnected crossings" within the industries. Highway and railroad officials had each received numerous complaints from the public about the insufficient timing of the warnings provided by the signals in the year prior to the crash, and citizens later told of situations with vehicles unable to clear the tracks in a timely manner.

The National Transportation Safety Board (NTSB) investigation found that, while the bus driver was not aware that a portion of the bus was on the tracks as she should have, the timing of signals was so insufficient that, even if she had identified the hazard as the train approached, she would have had to proceed against a red traffic signal into the highway intersection to have moved out of the train's path.

Legislation and re-engineering of interconnected crossings across the state of Illinois combined with greater awareness elsewhere resulted in efforts to help to prevent similar crashes from recurring. Informational decals were also added to Illinois school buses advising drivers of the length of each bus, since the substitute school bus driver was apparently unaware of the exact length of the bus she was driving. Other states have also embraced that and related aspects and incorporated them into their school bus driver training curriculum.The Fox River Grove crash stands as the worst crash involving a Metra train in its history, and one of the worst grade crossing crashes in U.S. history. At the crash site, the improved signaling system installed after the crash now protects the passing trains and motor vehicle traffic. Nearby is a small memorial to the seven high school students killed in the crash.