John Woodruff, American runner and colonel (b. 1915)
John Woodruff may refer to:

John Woodruff (athlete) (1915–2007), American middle-distance runner

John Woodruff (representative) (1826–1868), U.S. Representative from Connecticut

John Woodruff (director) (fl. 2000s–2010s), American actor and director

John I. Woodruff (1864–1962), American football coach and member of the Pennsylvania House of Representatives