Guale was a historic Native American chiefdom of Mississippian culture peoples located along the coast of present-day Georgia and the Sea Islands. Spanish Florida established its Roman Catholic missionary system in the chiefdom in the late 16th century.

During the late 17th century and early 18th century, Guale society was shattered by extensive epidemics of new infectious diseases and attacks by other tribes. Some of the surviving remnants migrated to the mission areas of Spanish Florida, while others remained near the Georgia coast. Joining with other survivors, they became known as the Yamasee, an ethnically mixed group that emerged in a process of ethnogenesis.