Mission San Luis Obispo de Tolosa (Spanish: Misión San Luis Obispo de Tolosa) is a Spanish mission founded September 1, 1772 by Father Junípero Serra in San Luis Obispo, California. Named after Saint Louis of Anjou, the bishop of Toulouse, the mission is the namesake of San Luis Obispo. The mission offers public tours of the church and grounds.

The Mission of San Luis Obispo is unusual in its design, in that its combination of belfry and vestibule are found nowhere else among the California missions. Like other churches, the main nave is short and narrow, but at the San Luis Obispo Mission, there is a secondary nave of almost equal size situated to the right of the altar, making it the only L-shaped mission church in California.