The Holocaust in Ukraine took place in the Reichskommissariat Ukraine, the General Government, Crimean General Government and some areas under military control to the East of Reichskommissariat Ukraine (all subdued to Nazi Germany), in the Transnistria Governorate and Northern Bukovina (both occupied with the latter annexed by Romania) and Carpathian Ruthenia (then part of Hungary) in World War II. The listed areas are today part of Ukraine. Between 1941 and 1944, more than a million Jews living in the Soviet Union were murdered by Nazi Germany's "Final Solution" extermination policies. Most of them were killed in Ukraine because most pre-WWII Soviet Jews lived in the Pale of Settlement, of which Ukraine was the biggest part.

According to Yale historian Timothy D. Snyder, "the Holocaust is integrally and organically connected to the Vernichtungskrieg, to the war in 1941, and is organically and integrally connected to the attempt to conquer Ukraine."