The Siege of Jerusalem (circa 589587 BCE) was the final event of the Judahite revolts against Babylon, in which Nebuchadnezzar II, king of the Neo-Babylonian Empire, besieged Jerusalem, the capital city of the Kingdom of Judah. Jerusalem fell after a 30-month siege, following which the Babylonians devastated the city and destroyed the First Temple. The Kingdom of Judah was dissolved and many of its inhabitants were exiled to Babylon.

During the late 7th century BCE, Judah became a vassal kingdom of Babylon. In 601 BCE, Jehoiakim, king of Judah, revolted against Babylonian rule. despite the strong remonstrances of the prophet Jeremiah. Jehoiakim died for reasons unclear, and was succeeded by his son, Jeconiah. In 597 BCE, the Babylonians besieged Jerusalem, and the city surrendered. Nebuchadnezzar pillaged Jerusalem and deported Jeconiah and other prominent citizens to Babylon; Jeconiah's uncle, Zedekiah, was installed as king. Later, encouraged by the Egyptians, Zedekiah launched a second revolt, and a Babylonian army was sent to retake Jerusalem.On Tisha B'Av, 25 August 587 BCE or 18 July 586 BCE, the Babylonians took Jerusalem, destroyed the First Temple and burned down the city. Zedekiah attempted escaping, but was captured near Jericho. In Riblah he was forced to watch his sons' death, and afterwards, his eyes were put out.The destruction of Jerusalem and its temple led to a religious, spiritual and political crisis, which left its mark in prophetic literature and biblical tradition. The Kingdom of Judah was abolished and annexed as a Babylonian province with its center in Mizpah. The Judean elite, including the Davidic dynasty, were exiled to Babylon. After Babylon had fallen to Cyrus the Great, founder of the Persian Achaemenid Empire, in 539 BCE, he allowed the exiled Judeans to return to Zion and rebuild Jerusalem. The Second Temple was completed in 516 BCE.

The Neo-Babylonian Empire, also known as the Second Babylonian Empire and historically known as the Chaldean Empire, was the last of the Mesopotamian empires to be ruled by monarchs native to Mesopotamia. Beginning with Nabopolassar's coronation as King of Babylon in 626 BC and being firmly established through the fall of the Neo-Assyrian Empire in 612 BC, the Neo-Babylonian Empire and its ruling Chaldean dynasty were short-lived, conquered after less than a century by the Persian Achaemenid Empire in 539 BC.

The defeat of the Assyrians and the transfer of empire to Babylon marked the first time the city, and southern Mesopotamia in general, had risen to dominate the Ancient Near East since the collapse of Hammurabi's Old Babylonian Empire nearly a thousand years earlier. The period of Neo-Babylonian rule thus saw unprecedented economic and population growth throughout Babylonia and a renaissance of culture and artwork, with the Neo-Babylonian kings conducting massive building projects, especially in Babylon itself, and bringing back many elements from the previous 2,000 or so years of Sumero-Akkadian culture.

The empire retains a position within modern day cultural memory mainly due to the unflattering portrayal of Babylon and its greatest king, Nebuchadnezzar II, in the Bible, which is owed to Nebuchadnezzar's 587 BC destruction of Jerusalem and the subsequent Babylonian captivity. Babylonian sources describe Nebuchadnezzar's reign as a golden age that transformed Babylonia into the greatest empire of its time.

Religious policies introduced by the Neo-Babylonian Empire's final king, Nabonidus, who favored the moon god Sîn over Babylon's patron deity Marduk, eventually provided a casus belli that allowed the Achaemenid king Cyrus the Great to invade Babylonia in 539 BC, portraying himself as a champion of Marduk divinely restoring order to the region. Babylon remained culturally distinct for centuries, with references to people with Babylonian names and references to the Babylonian religion known from as late as the Parthian period in the 1st century BC. Although Babylon revolted several times during the rule of later empires, it never successfully restored its independence.